Diversidad de comunidades fúngicas que habitan en tejido foliar sintomático de la uvilla Physalis peruviana en los Andes Ecuatorianos

Contenido principal del artículo

Wagner Chaves Acuña
José Luis Díaz
Jeniffer Yánez

Resumen

Utilizamos métodos cultivo-dependientes para evaluar la ocurrencia y diversidad de comunidades fúngicas que habitan en tejido foliar sintomático de uvilla (Physalis peruviana) en cuatro localidades del norte de los Andes ecuatorianos. Categorizamos los aislados basándonos en las caracterí­sticas morfológicas de los cultivos obtenidos in vitro, los cuales identificamos al secuenciar la región de ADNr ITS. En total, recuperamos un total de 75 hongos ascomycetos que comprendí­an 13 géneros y 20 taxa. Encontramos que la mayorí­a de hongos recuperados de tejidos necróticos o cloróticos pertenecí­an al género Alternaria. Otros taxa que encontramos con frecuencia fueron Epicoccum, Diaporthe y Xylaria. A nuestro conocimiento, este es el primer estudio en reportar la micobiota asociada a plantas de P. peruviana que presenten hojas con sí­ntomas de enfermedad, de la cual reportamos 2 géneros de hongos nuevos para el Ecuador. Esperamos que nuestros resultados provean información útil para futuros estudios sobre control biológico aplicado a cultivos comerciales en los trópicos.

Descargas

La descarga de datos todavía no está disponible.

Detalles del artículo

Cómo citar
1.
Acuña WC, Díaz J L, Yánez J. Diversidad de comunidades fúngicas que habitan en tejido foliar sintomático de la uvilla Physalis peruviana en los Andes Ecuatorianos . REMCB [Internet]. 18 de noviembre de 2019 [citado 28 de marzo de 2024];40(2):77-6. Disponible en: https://remcb-puce.edu.ec/remcb/article/view/762
Sección
Artículos Científicos

Citas

Altamirano M. 2010. Estudio de la cadena productiva de uvilla (Physalis peruviana L.) en la Sierra norte del Ecuador. Ecuador: Universidad San Francisco de Quito, Colegio de Agricultura, Alimentos y Nutrición, Quito.

Andersen RN, Walker HL. 1985. Colletotrichum coccodes: A pathogen of eastern black nightshade (Solanum ptycanthum). Weed Sci. 33: 902-905.

AnguloR,editor.2005.Uchuva:elcultivo.Colombia: Universidad de Bogotá Jorge Tadeo Lozano. 78 p.

Arnold AE, Lutzoni F. 2007. Diversity and host range of foliar endophytes: are tropical leaves biodiversity hotspots? Ecology. 88: 541-549.

Bruez E, Vallance J, Gerbore J, Lecomte P, Da Costa J, Guerin-Dubrana L, Rey P. 2014. Analyses of the temporal dynamics of fungal communities colonizing the healthy wood tissues of escaleaf- symptomatic and asymptomatic vines. PLoS One. 9: e95928.

Catal M. 2002. Development and testing of oligonucleotideprobesfordetectionandidentification ofsomefungal pathogensandendophytesofconifers. PhD dissertation. USA: Michigan State University, Department of Botany and Plant Pathology.

Cornejo-Espinoza FJ. 2014. Diversidad de hongos endófilos relacionados con lesiones necróticas en fréjol común (Phaseolus vulgaris L.) en la provincia de Loja, Ecuador. Bachelor’s thesis. Ecuador:PontificiaUniversidadCatólicadelEcuador.

Crous PW, Petrini O, Marais GF, Pretorius ZA, Rehder F. 1995. Occurrence of fungal endophytes in cultivars of Triticum aestivum in South Africa. Mycoscience. 36: 105-111.Crous PW, Verkley GJ, Groenewald E, Samson RA. 2009. CBS laboratory manual series 1: fungal biodiversity. The Netherlands,CentralBureauvoorSchimmelcultures.

Crozier J, Thomas SE, Aime MC, Evans HC, Holmes KA. 2006. Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao. Plant Pathol. 55: 783-791.

Dávila MF, Chaves-Acuña W, Yánez J. 2018. Fungal communities associated with symptomatic leaves of naranjilla (Solanum quitoense Lam.) in the Ecuadorian Amazon region. Rev Ecuat Med Cienc Biol. 39: 39-49.

De la Rotta, M.C. 2014. Enfermedades de la uchuva (Physalis peruviana L.). In: Carvalho CP, Moreno DA, editors. Physalis peruviana: fruta andina para el mundo. Spain: Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo – CYTED. p. 49-78.

Douanla-Meli C, Langer E, Mouafo FT. 2013. Fungal endophyte diversity and community patterns in healthy and yellowing leaves of Citrus limon. Fungal Ecol. 6: 212-222.

Evans HC, Reeder RH. 2000. Fungi associated with Eichhornia crassipes (water hyacinth) in the upper Amazon Basin and prospects for their use in biological control. In: Julien M, Hill MP, Center T, Ding J, editors. Proceedings of the Meeting of the Global Working Group for the Biological Control and Integrated Control of Water Hyacinth; Australia: Australian Centre for International Agricultural Research.

Fischer G. 2000. Ecophysiological aspects of fruit growingintropicalhighlands.ActaHortic.531:91-98.

Fischer G, Miranda D. 2012. Uchuva (Physalis peruviana L.). In: Fischer G, editor. Manual para el cultivo de frutales en eltrópicoColombia:Produmedios p. 851-873.

Fischer G, Almanza-Merchán P, Miranda D. 2014. Importancia y cultivo de la uchuva (Physalis peruviana L.). Rev Bras Frutic. 36: 1-15.

Gazis R, Chaverri P. 2010. Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru. Fungal Ecol. 3: 240- 254.

Ghimire SR, Charlton ND, Bell JD, Krishnamurthy YL, Craven KD. 2011. Biodiversity of fungal endophyte communities inhabiting switchgrass (Panicum virgatum L.) growing in the native tallgrass prairie of northern Oklahoma. Fungal Divers. 47: 19-27.

Gonzaga LL, Costa LEO, Santos TT, Araújo EF, Queiroz MV. 2015. Endophytic fungi from the genus Colletotrichum are abundant in the Phaseolus vulgaris and have high genetic diversity. J Appl Microbiol. 118: 485-496.

Hammer Ø, Harper DAT, Ryan PD. 2001. PAST: Paleontological statistics software package for educationanddataanalysis. Palaeontol Electron. 4: 9.

Herrmann LW, Poitevin CG, Schuindt LC, De Almeida AB, Pimentel IC. 2019. Diversity of fungal endophytes in leaves of Mentha piperita and Mentha canadensis. Int J Botany Stud. 4: 44- 49.

Hsieh HM, Lin CR, Fang MJ, Rogers JD, Fournier J, Lechat C, Ju YM. 2010. Phylogenetic status of Xylaria subgenus Pseudoxylaria among taxa of the subfamily Xylarioideae (Xylariaceae) and phylogeny of the taxa involved in the subfamily. Mol Phylogenetics Evol. 54: 957-969.

Hyde KD, Soytong K. 2008. The fungal endophyte dilemma. Fungal Divers. 33: 163-173.

Kaur A, Oberhofer M, Juzumaite M, Raja HA, Gulledge TV, Kao D, Faeth SH, Laster SM, Oberlies NH, Cech NB. 2016. Secondary metabolites from fungal endophytes of Echinacea purpurea suppress cytokine secretion by macrophage-type cells. Nat Prod Commun. 11:1143.

Kernaghan G, Mayerhofer M, Griffin A. 2017. Fungal endophytes of wild and hybrid Vitis leaves and their potential for vineyard biocontrol. Can J Microbiol. 63:583-95.

Kriel WM, Swart WJ, Crous PW. 2000. Foliar endophytes and their interactions with host plants, with specific reference to the Gymnospermae. In: Callow JA, editor. Advances in botanical research. London: Academic Press. p. 1-34.

Legge A. 1974. Notes on the history, cultivation and uses of Physalis peruviana L. J Roy Hort Soc. 99: 310-314.

Ling KS, Wechter WP, Somai BM, Walcott RR, Keinath AP. 2010. An improved real-time PCR system for broad-spectrum detection of Didymella bryoniae, the causal agent of gummy stem blight of cucurbits. Seed Sci Technol. 38: 692-703.

Loro M, Valero-Jiménez CA, Nozawa S, Márquez LM. 2012. Diversity and composition of fungal endophytes in semiarid Northwest Venezuela. J Arid Environ. 85: 46-55.

Maher N, Piot J, Bastien S, Vallance J, Rey P, Guérin-Dubrana L. 2012. Wood necrosis in esca- affected vines: types, relationships and possible links with foliar symptom expression. J Int Sci Vigne Vin. 46: 15-27.

Manamgoda DS, Rossman AY, Castlebury LA, Crous PW, Madrid H, Chukeatirote E, Hyde KD. 2014. The genus bipolaris. Stud Mycol. 79: 221-88.

Mazorra M. 2006. Aspectos anatómicos de la formación y crecimiento del fruto de uchuva Physalis peruviana (Solanaceae). Acta Biolo Colomb. 11: 69-81.

McCain R. 1993. Goldenberry, passionfruit and white sapote: potential fruits for cool subtropical areas. In Janick J, Simon JE, editors. New Crops. New York: Wiley and Sons. p. 479-486.

Miles LA, Lopera CA, González S, de García MC, Franco AE, Restrepo S. 2012. Exploring the biocontrol potential of fungal endophytes from an Andean Colombian Paramo ecosystem. BioControl. 57: 697-710.

Mondal SN, Vicent A, Reis RF, Timmer LW. 2007. Saprophytic colonization of citrus twigs by Diaporthe citri and factors affecting pycnidial production and conidial survival. Plant Dis. 91: 387-392.

Morton JF. 1987. Cape gooseberry. In: Morton JF, editor. Fruits of warm climates. Winterville, NC: Creative Resource Systems Inc. p. 430-434. Moya- Maldonado, A.E. 2016. Biodiversidad fúngica endófita y epífita de Citrus sinensis, naranjo dulce, de dos localidades de la Región Litoral del Ecuador. Bachelor’s thesis. Ecuador: Pontifica Universidad Católica del Ecuador.

Muñoz L. 2003. Análisis económico y financiero de la comercialización y producción de la uvilla como alternativa para la exportación. Bachelor’s thesis. Ecuador: Escuela Superior Politécnica del Litoral, Guayaquil.

Nilsson RH, Kristiansson E, Ryberg M, Hallenberg N, Larsson KH. 2008. Intraspecific ITS variability in the kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. Evol Bioinform. 4: 193-201.

Novoa R, Bojacá M, Galvis J, Fischer G. 2006. La madurez del fruto y el secado del cáliz influyen en el comportamiento poscosecha de la uchuva, almacenada a 12 °C (Physalis peruviana L.). Agron Colomb. 24: 77-86.

Pacin AM, Gonzalez HHL, Etcheverry M, Resnik SL, Vivas L, Espin S. 2003. Fungi associated with food and feed commodities from Ecuador. Mycopathologia. 156: 87-92.

Panno L, Bruno M, Voyron S, Anastasi A, Gnavi G, Miserere L, Varese GC. 2013. Diversity, ecological role and potential biotechnological applications of marine fungi associated to the seagrass Posidonia oceanica. N Biotechnol. 30: 685-94.

Paparu P, Dubois T, Gold CS, Niere B, Adipala E, Coyne D. 2008. Screenhouse and field persistence of nonpathogenic endophytic Fusarium oxysporum in Musatissuecultureplants.Microb Ecol. 55: 561-568.

Parsa S, García-Lemos AM, Castillo K, Ortiz V, López-Lavalle LAB, Braun J, Vega FE. 2016. Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris. Fungal Biol. 120: 783-790.

Petrini O. 1996. Ecological and physiological aspects of host specificity in endophytic fungi. In: Redlin SC, Carris LM, editors. Endophytic fungi of grasses and woody plants. St Paul, MN: American Phytopathological Society. p. 87-100.

Photita W, Lumyong S, Lumyong P, McKenzie EHC, Hyde KD. 2004. Are some endophytes of Musa acuminata latent pathogens? Fungal Divers. 16: 131-140.

Prihastuti H, Cai L, Chen H, Hyde KD. 2009. Characterization of Colletotrichum species associated with coffee berries in Chiang Mai, Thailand. Fungal Divers. 39: 89-109.

Promputtha L, Lumyong S, Dhanasekaran V, McKenzie E, Hyde K, Jeewon R. 2007. A phylogenetic evaluation of whether endophytes become saprotrophs at host senescense. Microb Ecol. 53: 579-590.

Puente LA, Pinto-Muñoz CA, Castro ES, Cortés M. 2011. Physalis peruviana Linnaeus, the multiple properties of a highly functional fruit: A review. Food Res Int. 44: 1733-1740.

Raja HA, Kaur A, El-Elimat T, Figueroa M, Kumar R, Deep G, Agarwal R, Faeth SH, Cech NB, Oberlies NH. 2015. Phylogenetic and chemical diversity of fungal endophytes isolated from Silybum marianum (L) Gaertn.(milk thistle). Mycology. 6: 8-27.

Ramadan MF, Mörsel JT. 2003. Oil goldenberry (Physalis peruviana L.). J Agric Food Chem. 51: 969-974.

Ramírez J, Fernandez ED, Rodolfi M, Solveig T. 2006. Actividad antagónica de hongos endófitos de plantas medicinales del Ecuador sobre bacterias patógenas. Bol Micol. 21: 49-53.

Rao VG, Subramoniam V. 1976. A new post-harvest diseaseofcape-gooseberry.JUnivBombay.72:58-61.

Rehm S, Espig G. 1991. Fruit. In: Sigmund R, Gustav E, editors. The cultivated plants of the tropics and subtropics, cultivation, economicvalue, utilization. Germany: Verlag Josef Margraf. p. 169- 245.

Rekab D, Del Sorbo G, Reggio C, Zoina A, Firrao G. 2004. Polymorphisms in nuclear rDNA and mtDNA reveal the polyphyletic nature of isolates of Phomopsis pathogenic to sunflower and a tight monophyletic clade of defined geographic origin. Mycol Res. 108:393-402.

Rocha DOL, Reis GM, da Silva VN, Braghini R, Teixeira MM, Correa B. 2011. Molecular characterization and fumonisin production by Fusarium verticillioides isolated from corn grains of different geographic origins in Brazil. Int J Food Microbiol. 145: 9-21.

Rodrigues A, Mueller UG, Ishak HD, Bacci Jr M, Pagnocca FC. 2011. Ecology of microfungal communities in gardens of fungus-growing ants (Hymenoptera: Formicidae): a year-long survey of three species of attine ants in Central Texas. FEMS Microbiol Ecol. 78: 244-55.

Rodríguez R, Redman R. 2008. More than 400 million years of evolution and some plants still can’t make it on their own: plant stress tolerance via fungal symbiosis. J Exp Bot. 59: 1109-1114.

Rodríguez-Amézquita JE, Velandia-Monsalve J, Viteri-Rosero SE. 2010. Evaluación de microorganismos aislados de gallinaza por su potencial para el biocontrol de fusarium (F. oxysporum) en plántulas de uchuva (Physalis peruviana). Rev Fac Nal Agr Medellín. 63: 5499- 5509.

Schulz B, Boyle C. 2006. What are endophytes? In: Schulz B, Boyle CJ, Sieber TN, editors. Microbial root endophytes. Berlin: Springer. p. 1-13. Shrestha P, Szaro TM, Bruns TD, Taylor JW. 2011. Systematic search for cultivatable fungi that best deconstruct cell walls of Miscanthus and sugarcane in the field. Appl Environ Microbiol. 77: 5490-5504.

Sieber T. 2007. Endophytic fungi in forest trees: are they mutualists? Fungal Biol Rev. 21: 75-89.

Stefańczyk E, Sobkowiak S, Brylińska M, Śliwka J. 2016. Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland. Eur J Plant Pathol. 145: 871-884.

Sun J, Guo L, Zang W, Ping W, Chi D. 2008. Diversity and ecological distribution of endophytic fungi associated with medicinal plants. Sci China C Life Sci. 51: 751-759.

Thomas SE, Crozier J, Aime MC, Evans HC, Holmes KA. 2008. Molecular characterization of fungal endophytic morphospecies associated with the indigenous forest tree, Theobroma gileri, in Ecuador. Mycol Res. 112: 852-860

Thomas DC, Vandegrift R, Ludden A, Carroll GC, Roy BA. 2016. Spatial ecology of the fungal genus Xylaria in a tropical cloud forest. Biotropica. 48: 381-393.

Van Bael SA, Fernández-Marín H, Valencia MC, Rojas EI, Wcislo WT, Herre EA. 2009. Two fungal symbioses collide: endophytic fungi are not welcome in leaf-cutting ant gardens. Proc R Soc Lond B Biol Sci. 276:2419-2426.

Videira SI, Groenewald JZ, Braun U, Shin HD, Crous PW. 2016. All that glitters is not Ramularia. Stud Mycol. 83: 49-163.

Waculicz-Andrade CE, Savi DC, Bini AP, Adamoski D, Goulin EH, Silva GJ, Massola NS, Terasawa LG, Kava V, Glienke C. 2017. Colletotrichum gloeosporioides sensu stricto: an endophytic species or citrus pathogen in Brazil?. Australas Plant Pathol. 46: 191-203.

Weir BS, Johnston PR, Damm U. 2012. The Colletotrichum gloeosporioides species complex. Stud Mycology. 73: 115-80.

White TF, Brunus T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsnky FS, White TT, editors. PCR protocol: a guide to methods and applications. San Diego: Academic Press. p. 315-322.

Zapata JL, Saldarriaga A, Londoño M, Díaz C. 2002. Manejo del cultivo de la uchuva en Colombia. Colombia: Corpoica. Boletin técnico.