Detección de genes codificantes de enzimas modificadoras de aminoglucósidos (EMAs) en aislados clínicos de Pseudomonas aeruginosa.

Abstract

Bacterial resistance to antibiotics represents a major public health issue. Pseudomonas aeruginosa constitutes a leading cause of nosocomial infections. The pathogenicity of P. aeruginosa is intensified by its intrinsic and acquired resistance to wide variety antibacterial agents. Acquired resistance spreads through genetic mobile elements such plasmids and transposons, leading to the emergence of multiresistant strains. Aminoglycosides represents a key family of antibacterials used to treat infections caused by P. aeruginosa. The aim of this study was to detect the presence of aminoglycoside-resistance genes in 78 resistant clinical isolates of P. aeruginosa from several hospitals in Quito-Ecuador. The specie confirmation was performed by amplifying the oprL gen. The most frequently reported AMEs genes in Pseudomonas aeruginosa: aac (3)-IIa, aac (6 ‘)-Ib, ant (2’’)-Ia, aph (3’)-VIa were detected by PCR using specific primers. AMEs genes were found in 64.10% of isolates. The most prevalent genes were aac (3)-IIa, aac (6’)-Ib and ant (2’’)-Ia. The most common profiles of AMEs genes were aac (3)-IIa, and aac (6 ‘)-Ib / ant (2’’) Ia, consistent with phenotypic resistance profiles.

This study allows us to gain better understanding of the resistance mechanisms related with genes that produce aminoglycoside modifying enzymes.