Búsqueda de bacterias con actividad EC 3.2.1.4 (endo-1,4- beta-glucanasa) en Eisenia foetida (Oligochaeta, Lumbricidae)

Abstract

The earth worm, Eisenia foetida, is host for numerous symbiotic microorganisms, many of which have been recently identified, as well as the description of their enzymatic activity.

These microorganisms usually present enzymatic activity of different type, and some may present industrial interest due to their potential applications. In the present research, it was decided to identify those microorganisms that present endo-β-1,4-glucanasa activity, and to evaluate this activity by the Nelson-Somogyi method of reducing sugars. Reducing sugars were released after the incubation of the bacteria colonies with carboxymethyl cellulose (CMC) solution. The isolated colonies of interest were cultured in laboratory and their identification was made through analysis of the 16S DNAr region. By comparing the results with the NCBI database, it was found that the endo-β-1,4- glucanase activity producing species were: Burkholderia fhytofirmans, Bacillus amyloliquefaciens, Bacillus licheniformis, and Acidovorax delafieldii. The isolations produced an interesting degradation percentage in relation to the positive control utilized. Comparisonsof the degradation caused by the different isolated colonies are presented.