In vitro characterization of Trichoderma spp., and mathematical model of its antagonism in the presence of Moniliophthora perniciosa
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Published:
May 30, 2017
Keywords:
Antagonist, cocoa, mathematical modelling, plant pathogen, sigmoid function
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Abstract
To optimization of practical use of the biological control agent is fundamental the understanding of how they were affected by the physics conditions, the survivor capacities, growths and reproduction systems. Trichoderma has been described as an efficient biological control agent. The aim of this research have been the in vitro characterization, determination of the growth of native strains of Trichoderma spp., from Ecuador and the esta-blishment of the antagonistic capacity of this isolates against M. perniciosa through the application of mathematical models. The research experiment was carried out in the Portoviejo Research Station (EEP) of Agricultural and Cattle National Research Institute (INIAP). Consequently, 12 isolates of Trichoderma spp., were identified based on charac-teristics of aroma, color and morphology. The isolated A1, which showed a strong smell of coconut, presented initial high growth ranges in pure culture and a flat morphology; was the most effective in the inhibition of M. perniciosadevelopment. The mathematical models determination describing the mycelial growth of M. perniciosa to the presen-ce of antagonistic isolates of Trichoderma spp., could allow a rapid characterization of Trichoderma spp., isolates in the praxis.
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Párraga Vélez J, Cañadas López Álvaro, Fajardo Navarrete P, Rade Loor D, Andrade Candell J. In vitro characterization of Trichoderma spp., and mathematical model of its antagonism in the presence of Moniliophthora perniciosa. REMCB [Internet]. 2017May30 [cited 2024Jul.3];38(1):25-4. Available from: https://remcb-puce.edu.ec/remcb/article/view/18
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Verma M, Brar S, Tyagi RD, Surampalli R, Valéro JR. 2007. Antagonist fungi, Trichoderma spp: Panoply of biological control. Biochemical Engineering Journal 37:1–20.
Bailey B, Bae H, Stem M, Crozier J, Thomas S, Samuels G, et al. 2008. Antibiosis, mycoparasitism, and colonization success for endophytic Trichoderma isolates with biological control potential in Theobroma cacao. Biological Control 46:24–35.
Begoude B, Lahlali R, Friel D, Tondje P, Jijakli M. 2007. Response surface methodology study of the combined effects of temperature, pH, and aw on the growth rate of Trichoderma asperellum. Journal of Applied Microbiology 103(4):845–54.
Bortz, J. 2010. Statistik für Sozialwissenschaftler. 7 Auflage. Springer-Verlag. Frankfurt-Deutschland. 882 p.
Cañadas A, Rade D, Domínguez JM, Murillo I, Molina C. 2016. Modelación Forestal como Innovación Tecnológica para el Manejo Silvicultural y Aprovechamiento Econó- mico de la Balsa, Región Costa-Ecuador. Ed. Abya Yala, Quito-Ecuador. 173 p.
Cañadas A, Vilčko F, Rade D, Zambrano C, Molina C. 2014. Hacia una descripción de fuste para Laurel (Cordia alliodora) en sistemas agroforestales, bosque protector Sumaco. Avances en Ciencias e Ingenierías 6(1):24–31.
De la Cruz M, Ortíz C, Bautista C, Ramírez J, Ávalos N, Cappello S, et al. 2015. Diversidad de Trichoderma spp., en el agroecosistema cacao del estado de Tabasco, México. Revista Mexicana de Biodiversidad 86:947–61.
Evans H, Holmes K, Thomas S. 2003. Endophytes and mycoparasites associated with an indigenous forest tree, Theobroma gileri, in Ecuador and a preliminary assessment of their potential as bio control agents of cocoa diseases. Mycological Progress 2(2):149–160.
Franzen M, Borgerhoof M. 2007. Ecological, economic and social perspectives on cocoa production worldwide. Biodiversity Conservation 16:3835–3849.
Guigón-López C, González-González P. 2004. Selección de cepas nativas de Trichoderma spp. con actividad antagónica sobre Phytophthora capsici Leonian y promotoras de crecimiento en el cultivo de chile (Capsicum annuum L.). Revista Mexicana de Fitopatología 22(1):1–9.
Hanada R, de Jorge Souza T, Pomella A, Hebbar K, Pereira J, Ismaiel A, et al. 2008. Trichoderma martiale sp.nov., a new endophyte from sapwood of Theobroma cacao with a potential for biological control. Mycological Research 112:1335–43.
Hernández A, Ruíz Y, Acebo Y, Miguélez Y, Heydrich M. 2014. Antagonistas microbianos para el manejo de la pudrición negra del fruto en Theobroma cacao L. Estado actual y perspectivas de uso en Cuba. Revista de Protección Vegetal 29(1):11–19.
Holmes K, Schroers H, Thomas S, Evans H and Samuels G. 2004, Taxonomy and bio control potential of a new species of Trichoderma from the Amazon basin of South America. Mycological Progress 3(3):199–210.
Lahlali R, Serrhini M, Jijakli M. 2005. Studying and modelling the combined effect of temperature and water activity on the growth rate of P. expansum. International Journal of Food Microbiology 103(3):315–322.
Larrea C, Warnars L. 2009. Ecuador´s Yasuní-ITT Initiative: Avoiding emissions by keeping petroleum underground. Energy for Sustainable Development 13:219–223.
Loor G, Fouet O, Lemainque A, Pavek S, Boccara M, Argout X, et al. 2013. Insight into the Wild Origin, migration and domestication history of the fine flavor national Theobroma cacao L. variety from Ecuador. Plos One 8(2):10.1371/annotation/2357f0f1-7dc3-4781-afb0-29a8ce- 56b3f0.
Melo C, Hollander G. 2013. Unsustainable development: Alternative food networks and the Ecuadorian Federation of Cacao Producers, 1995-2010. Journal of Rural Studies 32:251–263.
Nirenberg H. 1976. Untersuchungen über die morphologische und biologische Differenzierung in der Fusarium-Sektion Liseola. Mitt. Biol. Bundesanst. Land-u. Forstwirtsch. Berlin-Dahle 169:1–117.
Philips-Mora W, Aime M, Wilkinson M. 2007. Biodiversity and biogeography of the cacao (Theobroma cacao) pathogen Monilophthora roreri in tropical America. Plant Pathology 56:911–22.
Rivas M, Pavone D. 2010. Diversidad de Trichoderma spp., en plantas de Theobroma cacao L. del Estado Carabobo, Venezuela, y su capacidad de biocontroladora sobre Crinipellis perniciosa (STAHEL) SINGER. Interciencia 35(10): 777–783.
Rocha-Valdez J, Hassan M, Corkidi G, Flores C, Galindo E, Serrano-Carreón L. 2005. 6 pentyl α pyrone production by Trichoderma harzianum: The influence of energy dissipation rate and its implications on fungal physiology. Biotechnology and Bioengineering 91(1):54–61.
Ruf F. 2011. The myth of complex cocoa agro forests: the case of Ghana. Human Ecology 39:373–388.
Samuels GJ, Suarez, C, Solís K, Holmes K, Thomas S, Ismaiel A, et al. 2006. Trichoderma theobromicola and T. paucisporum: two new species isolated from cacao in South America. Mycological Research 110(4):381–392.
Samuels GJ. 2006. Trichoderma: systematics, the sexual state, and ecology. Phytopathology 96:196–206.
Verhulst P. 1838. Notice sur la loi que la population suit dans suit dans son accroissement. Correspondences Mathematiques et Physiques 10:113–121.
Verma M, Brar S, Tyagi RD, Surampalli R, Valéro JR. 2007. Antagonist fungi, Trichoderma spp: Panoply of biological control. Biochemical Engineering Journal 37:1–20.