Molecular sexing in Psitacidae
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Abstract
A high percentage of bird species are sexually monomorphic, so sexual determination is important both for breeding in captivity as well as for the establishment of conservation and management programs for endangered species. In the present work, a molecular sexing technique, initially described for the genus Ara, was applied to four other genera of Psittacidae: Pionus, Pionites, Amazona and Aratinga. The method consisted in the analysis of DNA restriction patterns generated by the HaeJII and DdeI enzymes in a region of the CHD 1 Z and CHD1 W genes. Individuals analyzed by this technique were mated and had offspring. The molecular sexing technique used in this study was efficient, fast, simple and non-aggressive for the individuals analyzed.
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