Crecimiento de oocitos biderianos en machos adultos de Rhinella marina (Linnaeus, 1758) mediante el uso de hormonas y disruptores endócrinos

Abstract

The Bidder’s organ is located anterior to the testis of some taxa of bufonid toads; it is a rudimentary non-functional ovary, which grows and partially develops if the testes are removed. In this research, we analyzed the growing effect of hormones and endocrine disruptors on Bidder’s organ (BO) oocytes of orchidectomized adult males of Rhinella marina. Adult males were orchidectomized and subjected to daily doses of saline, atrazine and progesterone solutions for a two months period. The effects of these compounds were tested by observations in the internal and external morphology and measurements of the maximum diameter of the Bidderian follicles.

All treatments showed an increase in the maximum diameter of bidderian oocytes after two months of treatment. Progesterone produced more advanced oogenesis stages than the ones with atrazine + progesterone. However, the number of grown oocytes in both cases was quite low. These results suggest that progesterone is the most suitable for inducing growth in the Bidderian oocytes of orchidectomized Rhinella marina male toads.