Molecular characterization of antimicrobial peptides from Agalychnis spurrelli (Anura: Hylidae) skin samples
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Abstract
The skin secretions of Agalychnis spurrelli have proven to have a strong antimicrobial activity on different pathogens because they contain certain biomolecules that have antimicrobial action. In the Phyllomedusinae family antimicrobial peptides are commonly present. Molecular analysis where performed in mRNA from the skin of A. spurrelli to determine the type of antimicrobial peptide present in the skin secretions of this species. The precursor that amplifies the sequences of mature peptides, where amplified by using specific primers that contained the highly conserved sequence, characteristic in this family. The result was a sequence of DNA complementary of 357 pb. This sequence was compared with their orthologs from other species of the same subfamily Phyllomedusinae, obtaining very high levels of identity for dermaseptin precursors. Finally, for the analysis of complementary DNA sequence, this was translated into an amino acid sequence. This sequence has the characteristics of peptides of the dermaseptin family: a highly conserved sequence, an acidic propiece with Lysine and Arginine and the variable the C-terminus. In conclusion, the total secretions of Agalychnis spurrelli contain a peptide of the family of dermaseptin or a dermaseptin like peptide.
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References
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