Marcadores de microsatélite de Rhodnius pallescens para análisis genéticos poblacionales en Rhodnius ecuadoriensis: Evaluación preliminar
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Published:
Nov 18, 2019
Keywords:
Chagas disease, Ecuador, microsatellites, Rhodnius
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Abstract
Rhodnius ecuadoriensis Lent & León (Hemiptera: Reduviidae) is a major Chagas disease (CD) vector in Ecuador, but little is known about its population genetic structure. We tested six Short Tandem Repeats (STRs) from R. pallescens Barber in wild and synanthropic R. ecuadoriensis populations. Two STRs were monomorphic, two yielded ambiguous alleles, and two were polymorphic (16 and 9 alleles) and used for analysis. Allele frequencies, AMOVA, and a Bayesian population structure test all favored a single-population hypothesis. These preliminary results suggest that local wild andsynanthropic R. ecuadoriensis populations frequently exchange migrants; long-term CD control will therefore require continuous entomological surveillance in coastal Ecuador
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1.
Grijalva MJ, Muñoz-Tobar SI, Abad-Franch F. Marcadores de microsatélite de Rhodnius pallescens para análisis genéticos poblacionales en Rhodnius ecuadoriensis: Evaluación preliminar. REMCB [Internet]. 2019Nov.18 [cited 2024Jul.3];40(2). Available from: https://remcb-puce.edu.ec/remcb/article/view/763
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Artículos Científicos
References
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Harry, M., Poyet, G., Romaña, C.A., Solignac, L. 1998. Isolation and characterization of microsatellite markers in the blood sucking bug Rhodnius pallescens (Heteroptera, Reduviidae). Molecular Ecology 7: 1771–1788.
Pritchard, J.K., Stephens, P., Donnelly, P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959.
Suárez-Dávalos, M.V., Dangles, O., Villacís, A.G., Grijalva, M.J. 2010. Microdistribution of sylvatic triatomine populations in central-coastal Ecuador. Journal of Medical Entomology 47: 80–88.
Villacís, A.G., Grijalva, M.J., Catalá, S.S. 2010. Phenotypic variability of Rhodnius ecuadoriensis populations at the Ecuadorian central and southern Andean region. Journal of Medical Entomology 47: 1034–1043.
Abad-Franch, F., Palomeque, F.S., Aguilar, H.M., Miles, M.A. 2005. Field ecology of sylvatic Rhodnius populations (Heteroptera, Triatominae): Risk factors for palm tree infestation in western Ecuador. Tropical Medicine & International Health10: 1258–1266.
Abad-Franch, F., Paucar, C. A., Carpio, C.C., Cuba Cuba, C.A., Aguilar, H.M., Miles, M.A. 2001. Biogeography of Triatominae (Hemiptera: Reduviidae) in Ecuador: implications for the design of control strategies. Memórias do Instituto Oswaldo Cruz 96: 611–620.
Abad-Franch, F., Vega, M.C., Rolón, M.S., Santos, W.S., Rojas de Arias, A. 2011. Community participation in Chagas disease vector surveillance: systematic review. PLoS Neglected Tropical Diseases 5: e1207.
Evanno, G., Regnaut, S., Goudet, J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14: 2611–2620.
Excoffier, L. Laval, G., Schneider, S. 2005:. Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1: 47–50.
Fitzpatrick, S., Feliciangeli, M.D., Sanchez-Martin M.J., Monteiro, F.A., Miles M.A. 2008. Molecular genetics reveal that silvatic Rhodnius prolixus do colonise rural houses. . PLoS Neglected Tropical Diseases 2: e210.
Grijalva, M.J., Palomeque-Rodríguez, F.S., Costales, J.A., Dávila, S., Arcos-Terán L. 2005. High household infestation rates by synanthropic vectors of Chagas disease in southern Ecuador.
Grijalva, M.J., Villacís, A.G., Ocaña-Mayorga, S., Yumiseva, C.A., Baus, E.G. 2011. Limitations of selective deltamethrin application for triatomine control in central coastal Ecuador. Parasites &Vectors, 4: 20.
Grijalva, M.J., Suarez-Davalos, V., Villacis, A.G., Ocana-Mayorga, S., Dangles, O. 2012. Ecological factors related to the widespread distribution of sylvatic Rhodnius ecuadoriensis populations in southern Ecuador. Parasites & Vectors 5: 17.
Grijalva, M. J., Terán, D., & Dangles, O. 2014. Dynamics of Sylvatic Chagas Disease Vectors in Coastal Ecuador Is Driven by Changes in Land Cover. PLoS Neglected Tropical Diseases 8(6):e2960.
Harry, M., Poyet, G., Romaña, C.A., Solignac, L. 1998. Isolation and characterization of microsatellite markers in the blood sucking bug Rhodnius pallescens (Heteroptera, Reduviidae). Molecular Ecology 7: 1771–1788.
Pritchard, J.K., Stephens, P., Donnelly, P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959.
Suárez-Dávalos, M.V., Dangles, O., Villacís, A.G., Grijalva, M.J. 2010. Microdistribution of sylvatic triatomine populations in central-coastal Ecuador. Journal of Medical Entomology 47: 80–88.
Villacís, A.G., Grijalva, M.J., Catalá, S.S. 2010. Phenotypic variability of Rhodnius ecuadoriensis populations at the Ecuadorian central and southern Andean region. Journal of Medical Entomology 47: 1034–1043.