Carbapenem resistance associated with class 1 integrons in Pseudomonas aeruginosa
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Abstract
Pseudomonas aeruginosa is a human opportunistic microbial agent commonly isolated from nosocomial infections. It is a difficult organism to treat due to its inherent resistance to various antibiotics and the rapid acquisition of resistance genes. In recent years, multiresistant isolates of P. aeruginosa with genes encoding enzymes that mainly confer resistance have been reported. to carbapenems: metallo-β-lactamases (MBL). These genes are often found in integrons. Its importance lies in the spread of resistance genes among Gram-negative bacteria. The aim of this study was to identify genes encoding metallo-β-lactamases (MBL) and their location in class 1 integrons in clinical isolates of P.aeruginosa. The 129 clinical isolates were collected at the Zurita&Zurita Laboratories between 2006 and 2008. The detection of genes that encode MBLs was performed by PCR, amplifying the genes mostly scattered among non-fermenting Gram-negative bacilli that produce MBLs: blaVIM, blaIMP, and blaSPM. The location of these genes in class 1 integrons was determined by nested PCR. Of the 129 isolates analyzed, 46 were resistant to carbapenems. Of the 46 resistant isolates, 34 (73.9%) had genes encoding MBL. Only 14.7% were located in class 1 integrons. The high prevalence of genes producing MBLs in the studied population was evident.
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1.
Yauri MF, Alcocer I, Zurita J, Rodríguez Riglos M. Carbapenem resistance associated with class 1 integrons in Pseudomonas aeruginosa. REMCB [Internet]. 2010Oct.1 [cited 2024Jul.3];31(1-2). Available from: https://remcb-puce.edu.ec/remcb/article/view/40
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References
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ANDRADE, V. 2005. Emergencia de la resistencia a carbapenemes en Pseudomonas aeruginosa productora de metalo β-lactamasas. Bio-quimia. 30: 52-58.
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CASTANHEIRA, M.; TOLEMAN,M.; JONES, R.; SCHMID, F.;WALSH, T. 2004. Molecular characterization of a β-lactamase gene, blaGIM-1enconding a new subclass of Metallo-b-lactamase. Antimicrobial agents and Chemotherapy. 48: 4654-4661.
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IYOBE, S.; YAMADA, H.; MINA-MI, S. 2006. Insertion of a carbapenemase gene cassette into an integron of a Pseudomonas aeruginosa plasmid. Journal Antimicrobial Chemotherapy. 39: 824-829.
XU, Z.; LI, L.; SHIRTLIFF, M.;ALAM, M.; YAMASAKI, S.; SHI,L. 2009. Occurrence and Characteristics of Class 1 and 2 Integrons in Pseudomonas aeruginosa isolates from patients in Southern China. Journal of Clinical Microbiology. 47: 230-234.
XU, J.; MOORE, J.; MURPHY, P.; MILLAR, C.; ELBORN, J. 2006. Early detection of Pseudomonas aeruginosa comparison of conventional versus molecular (PCR)detection directly from adult patients with cystic fibrosis (CF).Annals of Clinical Microbiology and Antimicrobials. 3: 21-25.
PITOUT, J.; GREGSON, D.; POI-REL, L. 2005. Detection of pseudomonas aeruginosa producing Metallo-β-Lactamases in a large centralized laboratory. Journal of Clinical Microbiology. 43:3129 – 3135.
SIARKOU, V.; VITTI, D.; PRO-TONOTARIOU, E.; IKONOMI-DIS, A.; SOFIANOU, D. 2009.Molecular epidemiology of Outbreak related Pseudomonas aeruginosa strains carrying the novel variant blaVIM-17Metallo−β-lac-tamase gene. Antimicrobial Agents and Chemotherapy. 53: 1325-1330.
GOOTZ, T. 2010. The global problem of antibiotic resistance. Critical Reviews in Immunology. 30:79-93.
GIBB, A.; TRIBUDDHARAT, R.; MOORE, T.; LOUIE, W.; KRULICKI, D.; LIVERMORE, M.; PALEPOU, F.; WOODFORD,N. 2002. Nosocomial outbreak of carbapenem-resistant Pseudomonas aeruginosa with a new blaIMP allele, blaIMP-7.Antimicrobial Agents and Chemotherapy. 46: 255-258.
ROSENTHAL, D.; MAKI DG, L.;MEHTA, A.; ALVAREZ-MORENO, C.; HIGUERA,F.;CUELLAR, L.; MADANI, N .2008. International Nosocomial Infection Control Consortium report, data summary for 2002-2007, issued January 2008. America Journal of Infection Control.36: 627-637.
CANO, M.; DOMÍNGUEZ, M.;EZPELETA, C.; PADILLA, B.;RAMÍREZ, E.; MARTÍNEZ, L.2008. Cultivos de vigilancia epidemiológica de bacteriasresistentes a los antimicrobianos deinterés nosocomial. Enfermedades Infecciosa y Microbiología Clínica.26: 220-229.
RAHAL, J. 2008. The role of carbapenems in initial therapy for serious Gram negative infections. BioMed Central LTD.12 (Suppl 4).
OLIVER, A. 2009. Impacto de la diseminación de Pseudomonas aeruginosa multiresistente productora de metalo−β-lactamasas en los hospitales: presente y futuro. Enfermedades Infecciosas y Microbiología Clínica. 27: 255-256.
PFEIFER, Y.; CULLIK, A.;WITTE, W. 2010. Resistance tocephalos porins and carbapenems in Gram negative bacterial pathogens. International Journal of Medical Microbiology. 34: 235-240.
ANDRADE, V. 2005. Emergencia de la resistencia a carbapenemes en Pseudomonas aeruginosa productora de metalo β-lactamasas. Bio-quimia. 30: 52-58.
WALSH, T.; TOLEMAN, M.;POIREL, L.; NORDMANN, P.2005. Metallo−β-lactamase: the Quiet before the Storm? Clinical Microbiology Reviews.18: 306-325.
AUBRON, C.; POIREL, L.; FORTINEAU, N.; NICOLAS, P.; COLLET, L.; NORDMANN, P. 2005.Nosocomial spread of Pseudomonas aeruginosa isolates expressing the metallo-β-lacta-mase VIM-2 in a hematology unit of a French hospital. Microbial Drug Resistance.11:254-259.
PAGNIEZ, G.; RADICE, M.; CUIROLO,A.;RODRÍGUEZ,O.; RODRÍGUEZ,H.; CAY,C.; FAMIGLIETTI, A.; GUTKIND, G. 2006. Prevalencia de metalolactamasas a carbapenemes en un hospital Universitario de Buenos Aires. Revista Argentina de Microbiología. 38: 33-37
GILLINGS, M.; BOUCHER, Y.; LABBATE, M.; HOLMES, A.; KRISHNAN, S.; HOLLEY, M.; STOKES, W. 2008. The Evolution of Class 1 Integrons and the Rise of Antibiotic resistance Journal of Bacteriology. 190: 5095-5100.
LOLANS, K.; QUEENAN, A.; BUSH, K.; SAHUD, A.; QUINN,J. 2005. First Nosocomial outbreak of Pseudomonas aeruginosa producing an Integronborne Metallo-−β-lactamase (VIM-2) in the United States. Journal of Antimicrobial Chemotherapy. 49: 3538-3540.
SÁNCHEZ, D.; MARCANO, D.;SPADOLA, E.; LEÓN, L.;PAYARES, D.; UGARTE, C.;SALGADO, N.; GUEVARA, A.;TORRES, S.; RODRÍGUEZ, J.;FLORES, R. 2008. Metaloenzimastipo VIM detectadas en aislamien-tos clínicos en Pseudomonas aeruginosa en cuatro hospitales en Venezuela. Revista del Instituto Nacional de Higiene Rafael Rangel. 39 (2).
OSANO, E.; ARAKAWA, Y.;PORN, R.; OHTA, M. 2004. Molecular Characterization of an Enterobacterial Metallo−β-lactamase found in a clinical isolate of Serratia marcescens that shows Imipenem resistance. Antimicrobial Agents and Chemotherapy. 38: 71-78.
LAURETTI, L.; RICCIO, A.;MAZZARIOL, G.; CORNAGLIA,G.; AMICOSANTE, R.;FONTANA, R.; ROSSOLINI, G.2000. Cloning and Characterization of bla VIM, a new integron born emetallo-β-lactamase gene from Pseudomonas aeruginosa clinical isolate. Journal of Antimicrobial Chemotherapy. 43: 1584-1590.
CASTANHEIRA, M.; TOLEMAN,M.; JONES, R.; SCHMID, F.;WALSH, T. 2004. Molecular characterization of a β-lactamase gene, blaGIM-1enconding a new subclass of Metallo-b-lactamase. Antimicrobial agents and Chemotherapy. 48: 4654-4661.
MAGALHAES, V.; LINS, A.;MAGALHAES, M. 2005. Metallo-b-lactamase-producing Pseudomonas aeruginosa trains isolated in Hospitals in Recife, PE, Brazil. Brazilian Journal of Microbiology. 36: 123-125.
IYOBE, S.; YAMADA, H.; MINA-MI, S. 2006. Insertion of a carbapenemase gene cassette into an integron of a Pseudomonas aeruginosa plasmid. Journal Antimicrobial Chemotherapy. 39: 824-829.
XU, Z.; LI, L.; SHIRTLIFF, M.;ALAM, M.; YAMASAKI, S.; SHI,L. 2009. Occurrence and Characteristics of Class 1 and 2 Integrons in Pseudomonas aeruginosa isolates from patients in Southern China. Journal of Clinical Microbiology. 47: 230-234.
XU, J.; MOORE, J.; MURPHY, P.; MILLAR, C.; ELBORN, J. 2006. Early detection of Pseudomonas aeruginosa comparison of conventional versus molecular (PCR)detection directly from adult patients with cystic fibrosis (CF).Annals of Clinical Microbiology and Antimicrobials. 3: 21-25.
PITOUT, J.; GREGSON, D.; POI-REL, L. 2005. Detection of pseudomonas aeruginosa producing Metallo-β-Lactamases in a large centralized laboratory. Journal of Clinical Microbiology. 43:3129 – 3135.
SIARKOU, V.; VITTI, D.; PRO-TONOTARIOU, E.; IKONOMI-DIS, A.; SOFIANOU, D. 2009.Molecular epidemiology of Outbreak related Pseudomonas aeruginosa strains carrying the novel variant blaVIM-17Metallo−β-lac-tamase gene. Antimicrobial Agents and Chemotherapy. 53: 1325-1330.
GOOTZ, T. 2010. The global problem of antibiotic resistance. Critical Reviews in Immunology. 30:79-93.
GIBB, A.; TRIBUDDHARAT, R.; MOORE, T.; LOUIE, W.; KRULICKI, D.; LIVERMORE, M.; PALEPOU, F.; WOODFORD,N. 2002. Nosocomial outbreak of carbapenem-resistant Pseudomonas aeruginosa with a new blaIMP allele, blaIMP-7.Antimicrobial Agents and Chemotherapy. 46: 255-258.