Characterization of the variable region of class 1 integrons in clinical isolates of Klebsiella pneumoniae resistant to carbapenems
Article Sidebar
Published:
Nov 30, 2016
Keywords:
Antibiotic resistance, carbapenems, class 1 integrons, gene cassette, Klebsiella pneumoniae
Main Article Content
Abstract
Integrons are structures frequently observed in Gram negative bacilli, especially among members of the Enterobacteriaceae family. The presence of class 1 integron and the relationship with bacterial resistance were studied. The integron presence was investigated by polymerase chain reaction (PCR) and sequencing. Thirty carbapenem-resistant Klebsiella pneumoniae isolates were analyzed and were positive for the intI1gene. Twenty six of these isolates amplified variable regions about 2000 base pairs. The common pattern of genes was (dfrA12-OrfF-aadA2). The presence of these platforms that promote genetic diversity gives resistance a broad range of antibiotics. The higher integron prevalence in this isolates functions as source for dissemination of resistance determinants.
Downloads
Download data is not yet available.
Article Details
How to Cite
1.
Yauri MF, Alcocer I, Rodríguez-Riglos M. Characterization of the variable region of class 1 integrons in clinical isolates of Klebsiella pneumoniae resistant to carbapenems. REMCB [Internet]. 2016Nov.30 [cited 2024Jul.3];37(2). Available from: https://remcb-puce.edu.ec/remcb/article/view/5
Section
Notas científicas
References
Brat S, Tulane P, Karumudi U, Quale J, et al. 2010. Car-bapenemase-producing Klebsiella pneumo-nia in Brooklyn, NY: molecular epidemiolo-gy and in vitro activity of polymyxin B and other agents. Journal of Antimicrobial Che-motherapy 56: 128-132.
Clinical and Laboratory Standards Institute. 2015. Per-formance Standards for Antimicrobial Sus-ceptibility Testing: Twenty-Fifth Informatio-nal Supplement. M100-S2
Conza D, Gutkind G. 2010. Integrones: los coleccionis-tas de genes. Revista Argentina de Microbio-logía 42: 63-78.
European Committee on Antimicrobial Susceptibility Testing. 2014. Breakpoint tables for interpre-tation of MICs and zone diameters. Versión 4.0.
Galdiero S, Falanga A, Cantisani M., Tarallo R, Pepa MED, D’Oriano V and Galdiero, M. 2012. Mi-crobe-Host Interactions: Structure and Role of Gram-Negative Bacterial Porins. Current Protein & Peptide Science 13(8), 843–854. http://doi.org/10.2174/138920312804871120
Hou, X., Song, X., Ma, X., Zhang, S., & Zhang, J. 2015. Molecular characterization of multi-drug-resistant Klebsiella pneumoniae isola-tes. Brazilian Journal of Microbiology 46(3), 759–768. http://doi.org/10.1590/S1517-838246320140138
Khoramrooz, S., Sharifi, A., Yazdanpanah, M., Hossei-ni, S., Emaneini, M., Gharibpour, F., Khos-ravani, S. 2016. High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Ya-suj, Iran. Iranian Red Crescent Medical Jour-nal 18(1):e26399.
Li B, Hu Y, Wang Q, Yi Y, Woo P, Jing H and Liu C. 2013. Structural Diversity of Class 1 Inte-grons and Their Associated Gene Cassettes in Klebsiella pneumoniae Isolates from a Hospi-tal in China. PLoS ONE 8(9): e75805.
Márquez C, Ingold A, Echeverría N, Acevedo A, Vig-noli R, García-Fulgueiras V and Galiana A. 2014. Emergence of KPC-producing Klebsie-lla pneumoniae in Uruguay: infection control and molecular characterization. New Micro-bes and New Infections 2(3), 58–63. http:// doi.org/10.1002/nmi2.40
Martínez-Martínez L and Calvo J. 2010. El proble-ma creciente de la resistencia antibiótica en bacilos Gram negativos: situación actual. Enfermedades Infecciosas y Microbiología Clínica 28, Supplement 2, 25–31. http://doi. org/10.1016/S0213-005X(10)70027-6
Martínez-Romero E, Garza-Ramos U and Silva-Sánchez J. 2009. Genética y genómica enfocadas en el estudio de la resistencia bacteriana. Salud Pública de México 51: 439-446.
Pacheco R, Osorio L, Correa A y Villegas M. 2014. Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia. Centro Internacional de Entrena-miento e Investigaciones. Biomédica 34(1): 81-90.
Pitout J, Gregson D and Poirel L. 2005. Detection of Pseudomonas aeruginosa producing Meta-llo-β-Lactamases in a large centralized labo-ratory. Journal of Clinical Microbiology 43(7): 3129-3135.
hcheraghi F, Rahmati G, Nobari S, Torabi E, Mou-savi S, Aslani FM and Shahcheraghi F. 2014. Identification and characterization of class 1 integrons among atypical enteropathogenic Escherichia coli isolated from children under 5 years of age. Iranian Journal of Microbio-logy 6(3), 156–162.
Vo A, van Duijkere, E, Gaastra W and Fluit A. 2010. An-timicrobial Resistance, Class 1 Integrons, and Genomic Island 1 in Salmonella Isolates from Vietnam. PLoS ONE 5(2), e9440.
Wan M and Chou Ch. 2015. Class 1 Integrons and the Antiseptic Resistance Gene (qacEΔ1) in Mu-nicipal and Swine Slaughterhouse Wastewa-ter Treatment Plants and Wastewater—Asso-ciated Methicillin-Resistant Staphylococcus aureus. International Journal of Environ-mental Research and Public Health 12: 6249-6260.
Yigit H, Queenan A, Anderson M, Domenech-Sanchez G, Biddle A, Steward J and Tenover F. 2001. Novel Carbapenem-Hydrolyzing β-Lactama-se, KPC-1, from a Carbapenem - Resistant Strain of Klebsiella pneumoniae. Antimicro-bial Agents and Chemotherapy 45(4), 1151– 1161. http://doi.org/10.1128/AAC.45.4.1151-1161.2001
Clinical and Laboratory Standards Institute. 2015. Per-formance Standards for Antimicrobial Sus-ceptibility Testing: Twenty-Fifth Informatio-nal Supplement. M100-S2
Conza D, Gutkind G. 2010. Integrones: los coleccionis-tas de genes. Revista Argentina de Microbio-logía 42: 63-78.
European Committee on Antimicrobial Susceptibility Testing. 2014. Breakpoint tables for interpre-tation of MICs and zone diameters. Versión 4.0.
Galdiero S, Falanga A, Cantisani M., Tarallo R, Pepa MED, D’Oriano V and Galdiero, M. 2012. Mi-crobe-Host Interactions: Structure and Role of Gram-Negative Bacterial Porins. Current Protein & Peptide Science 13(8), 843–854. http://doi.org/10.2174/138920312804871120
Hou, X., Song, X., Ma, X., Zhang, S., & Zhang, J. 2015. Molecular characterization of multi-drug-resistant Klebsiella pneumoniae isola-tes. Brazilian Journal of Microbiology 46(3), 759–768. http://doi.org/10.1590/S1517-838246320140138
Khoramrooz, S., Sharifi, A., Yazdanpanah, M., Hossei-ni, S., Emaneini, M., Gharibpour, F., Khos-ravani, S. 2016. High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Ya-suj, Iran. Iranian Red Crescent Medical Jour-nal 18(1):e26399.
Li B, Hu Y, Wang Q, Yi Y, Woo P, Jing H and Liu C. 2013. Structural Diversity of Class 1 Inte-grons and Their Associated Gene Cassettes in Klebsiella pneumoniae Isolates from a Hospi-tal in China. PLoS ONE 8(9): e75805.
Márquez C, Ingold A, Echeverría N, Acevedo A, Vig-noli R, García-Fulgueiras V and Galiana A. 2014. Emergence of KPC-producing Klebsie-lla pneumoniae in Uruguay: infection control and molecular characterization. New Micro-bes and New Infections 2(3), 58–63. http:// doi.org/10.1002/nmi2.40
Martínez-Martínez L and Calvo J. 2010. El proble-ma creciente de la resistencia antibiótica en bacilos Gram negativos: situación actual. Enfermedades Infecciosas y Microbiología Clínica 28, Supplement 2, 25–31. http://doi. org/10.1016/S0213-005X(10)70027-6
Martínez-Romero E, Garza-Ramos U and Silva-Sánchez J. 2009. Genética y genómica enfocadas en el estudio de la resistencia bacteriana. Salud Pública de México 51: 439-446.
Pacheco R, Osorio L, Correa A y Villegas M. 2014. Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia. Centro Internacional de Entrena-miento e Investigaciones. Biomédica 34(1): 81-90.
Pitout J, Gregson D and Poirel L. 2005. Detection of Pseudomonas aeruginosa producing Meta-llo-β-Lactamases in a large centralized labo-ratory. Journal of Clinical Microbiology 43(7): 3129-3135.
hcheraghi F, Rahmati G, Nobari S, Torabi E, Mou-savi S, Aslani FM and Shahcheraghi F. 2014. Identification and characterization of class 1 integrons among atypical enteropathogenic Escherichia coli isolated from children under 5 years of age. Iranian Journal of Microbio-logy 6(3), 156–162.
Vo A, van Duijkere, E, Gaastra W and Fluit A. 2010. An-timicrobial Resistance, Class 1 Integrons, and Genomic Island 1 in Salmonella Isolates from Vietnam. PLoS ONE 5(2), e9440.
Wan M and Chou Ch. 2015. Class 1 Integrons and the Antiseptic Resistance Gene (qacEΔ1) in Mu-nicipal and Swine Slaughterhouse Wastewa-ter Treatment Plants and Wastewater—Asso-ciated Methicillin-Resistant Staphylococcus aureus. International Journal of Environ-mental Research and Public Health 12: 6249-6260.
Yigit H, Queenan A, Anderson M, Domenech-Sanchez G, Biddle A, Steward J and Tenover F. 2001. Novel Carbapenem-Hydrolyzing β-Lactama-se, KPC-1, from a Carbapenem - Resistant Strain of Klebsiella pneumoniae. Antimicro-bial Agents and Chemotherapy 45(4), 1151– 1161. http://doi.org/10.1128/AAC.45.4.1151-1161.2001