Bactericidal activity of the colloidal silver water upon microbiological quality indicators: an analysis from environmental samples
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Abstract
The aim of this study was to evaluate the bactericidal activity of colloidal silver on microorganisms classified as indicators of microbiological quality which were isolated from samples of: (i) water of 16 irrigation canals in the province of Tungurahua – Ecuador and (ii) utensils and supplies from the kitchen in a cafe located in Quito – Ecuador. For this, silver water with a concentration of 4 ppm, 10 ppm, or 15 ppm was applied during 15, 30 or 60 minutes on cell suspensions equivalent to A625 between 0,08 – 0,1 of the strains: Escherichia coli ATCC 11229, Salmonella Choleraesuis ATCC 10708, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442. After the standardization of the technique, we compared the population density of: aerobic mesophilic bacteria, E. coli – coliform and Enterobacteriaceae in 16 samples of irrigation canal water and 11 samples of food preparation surfaces, before and after exposure to 4 ppm, 10 ppm or 15 ppm of colloidal silver. Regarding the irrigation canal water, the treatments inhibited the growth of all the evaluated microbial groups except in 31,3% (n=5) of the cases where exposure to 4 ppm of colloidal silver resulted in aerobic mesophilic bacteria counts ranging between 10 x 102 CFU/mL and 40 x 102 CFU/mL. In the case of samples collected in the cafe, the mesophilic bacteria counts decreased in 64,3% (n=9) of the total sampled surfaces, whereas counting of E. coli – coliform decreased in 93% (n=13) of the sampled areas after 5 minutes of exposure to 4ppm of colloidal silver.
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