Characterization of the amylase activity present in larval extracts of two potato pest moths: Tecia solanivora and Symmetrischema tangolias
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Abstract
Tecia solanivora and Symmetrischema tangolias (Lepidoptera: Gelechiidae) moths cause serious damage to Solanum tuberosum tubercles. The purpose of this study was to isolate and biochemically characterize amilases present in different larval moth stages. Soluble proteins were extracted from the larval stages raised in the laboratory. Differences in protein concentration were determined by spectrophotometry. Average weight for each larvae was calculated. T. solanivora was heavier and more soluble protein was extracted as compared with S. tangolias. Amilase activity of the different protein extracts was identified using starch degradation techniques. The amylasic activity of the IV stage of T. solanivora and S. tangolias incubated at different intervals of time were found to react different, however the results became similar when the activity of both extracts was scored after 72 h time reaction. Eleven protein bands between 225 and 10 kDa were identified electrophoretically. Among these bands, amylases were identified in both species in the 4 different stages in accordance to the molecular weights equivalent to 50 kDa. The protein bands of this enzyme were more defined in the I and II than in the III and IV stages, where they appeared quite diffused. Amylase activity was not linked to soluble protein concentrations; instead, it depended on reaction conditions. These results suggest the possibility of designing new forms of biocontrol against potato moths
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